Thorsten Naserke - Skills

My Skills


  • Cloning of RCAS retroviral expression constructs and production of virus
  • Primary culture and transfection of sympathetic neuroblasts
  • Growth factor treatment of schiatic nerve Schwann cells
  • DNA isolation from the Bursa of Fabricius
  • Breeding and maintenance of colonies
  • Genotyping
  • Dissection of timed embryos
  • Perfusion and preparation of postnatal brains
  • Immunocyto- and Immunohistochemistry
  • Radioactive in-situ hybridization on sections
  • Non-radioactive in-situ hybridization on whole mounts
Cell culture
  • Culture of chicken embryonic fibroblasts and DF-1 cells for retrovirus production
  • Antibody production from AMV-3C2 cells
  • Primary culture of chicken sympathetic neuroblasts and Schwann cells
Protein Biochemistry
  • Western Blotting
  • Immunoprecipitation
Molecular Biology
  • RNA/DNA isolation and purification
  • PCR, semiquantitative RT-PCR and Real-time PCR
  • Southern Blotting
  • Cloning